Direct Production of 7-Aminocephalosporanic Acid and 7-Aminodeacetyl-cephalosporanic Acid by Recombinant Acremonium chrysogenum.

Preparation of 7-aminocephalosporanic acid and 6-aminopenicillanic acid.

Possibilities to acylate 7-aminocephalosporanic acid to obtain some cephalosporins.

AIM Acylation of 7-aminocephalosporanic acid with an adequate acyl chloride in order to obtain cefotaxime sodium salt. MATERIAL AND METHODS Cefotaxime sodium salt was synthesized by acylating 7-amino cephalosporanic acid with 2-[2'-chloracetamidothiazole-4-yl]-2-(syn)-methoxy-imino acetic chloride in four steps. The melting point was determined, and IR spectral analysis and elemental analysis...

Biochemical characterization of a glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain BL072.

Pseudomonas strain BL072 produces an acylase enzyme active in hydrolyzing glutaryl-7-aminocephalosporanic acid to 7-aminocephalosporanic acid. This acylase was purified by column chromatography and gel electrophoresis. The native acylase was composed of two subunits of approximately 65 and 24 kDa, though some heterogeneity was seen in both the native acylase and its small subunit. The isoelectr...

Isolation and characterization of a pseudomonas strain producing glutaryl-7-aminocephalosporanic Acid acylase.

Several screening methods were developed for the selection of Pseudomonas strains capable of hydrolyzing glutaryl-7-aminocephalosporanic acid to 7-aminocephalosporanic acid. An isolate exhibiting high acylase activity, designated BL072, was identified as a strain of Pseudomonas diminuta. It grew optimally at pH 7 to 8 and at a temperature of 32 to 40 degrees C, but acylase activity was highest ...

Struvite precipitation for ammonia nitrogen removal in 7-aminocephalosporanic acid wastewater.

7-Aminocephalosporanic acid wastewater usually contains high concentrations of ammonium (NH₄⁺-N), which is known to inhibit nitrification during biological treatment processes. Chemical precipitation is a useful technology to remove ammonium from wastewater. In this paper, the removal of ammonium from 7-aminocephalosporanic acid wastewater was studied. The optimum pH, molar ratio, and various c...